Rapid Identification of Aspergillus Fumigatus Using Βeta-Tubulin and RodletA Genes
نویسندگان
چکیده
AIM The main purpose of the present study was to test the β-tubulin and rodletA genes for rapid identification of Aspergillus fumigatus. MATERIALS AND METHODS Fifty-one A. fumigatus strains including environmental, clinical and reference isolates were tested in this research. PCR was carried out based on βtub and rodA partial gene sequences. RESULTS A 198 bp DNA fragment was obtained using βtub gene. PCR amplification of the rodA gene resulted in a 313 bp band. The βtub and rodA genes PCR products exhibited a 100% homology with the associated sequences in the GenBank. CONCLUSION In the present study, we used a PCR approach that was able to discriminate A. fumigatus from other related species within the section Fumigati.
منابع مشابه
شناسایی مورفولوژیک و مولکولی آسپرژیلوسهای جداشده از بیماران بر اساس تعیین توالی ژن بتا توبولین (β-tubulin)
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